BAGATOLLI LUIS ALBERTO
Capítulos de libros
Título:
The Use of 6-Acyl-2-(Dimethylamino) Naphthalenes as Relaxation Probes of Biological Environments
Autor/es:
LUIS A. BAGATOLLI; D. JAMESON
Libro:
Perspectives on Fluorescence: A Tribute to Gregorio Weber
Editorial:
Springer International Publishing
Referencias:
Año: 2016;
Resumen:
As Gregorio Weber anticipated in his seminal 1979 article, 6-acyl-2-
(dimethylamino)naphthalene probes became excellent tools to study
nanosecond relaxation processes of biological systems. Examples are the
use of PRODAN (or DANCA) to study relaxation of specific protein
matrixes, or LAURDAN (as well as PRODAN) extensively used to study
the extent of water dipolar relaxation processes in biological membranes. In
this chapter a novel application for this family of molecules is presented and
discussed. Specifically, we show how these fluorescent probes can be used to
monitor intracellular water dipolar relaxation in living cells displaying
oscillatory metabolism. Our experimental results show a strong coupling
between metabolism and intracellular water dynamics, challenging the
view that water in the interior of cells exists mostly as a medium whose
global properties are comparable to the properties of dilute solutions. The
observed results can be very well interpreted in light of the associationinduction
hypothesis postulated by Gilbert Ling in 1962.
(dimethylamino)naphthalene probes became excellent tools to study
nanosecond relaxation processes of biological systems. Examples are the
use of PRODAN (or DANCA) to study relaxation of specific protein
matrixes, or LAURDAN (as well as PRODAN) extensively used to study
the extent of water dipolar relaxation processes in biological membranes. In
this chapter a novel application for this family of molecules is presented and
discussed. Specifically, we show how these fluorescent probes can be used to
monitor intracellular water dipolar relaxation in living cells displaying
oscillatory metabolism. Our experimental results show a strong coupling
between metabolism and intracellular water dynamics, challenging the
view that water in the interior of cells exists mostly as a medium whose
global properties are comparable to the properties of dilute solutions. The
observed results can be very well interpreted in light of the associationinduction
hypothesis postulated by Gilbert Ling in 1962.
