A model for Parkinson Disease: administration of rotenone by using microvesicles

BRUERA MANUEL; SANCHEZ SUSANA INES; ARCE MARIA ELENA; CIUFFO GLADYS MARIA

Córdoba

Congreso; XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2019

Sociedad Argentina de Investigación en Neurociencias

Parkinson Disease (PD) is one of the most frequent neurological diseases in elderness. Rotenone is an herbicide known to produce neurotoxic effects. Several methods of delivery have been explored, some of them with high mortality. Thus, we decided to administer rotenone by using microvesicles of a copolymer of PLGA. Microvesicles allow a slow delivery of the drug and thus a long treatment with a single dose administration. Resuspended microvesicles (25 um) were administered by subcutaneous injection in a dose of 50 mg/kg. Rats were weighted every day and no significant difference with control animals was observed during the whole treatment at the dose assayed. Animal´s behavior was evaluated by using the bar test, grid test and rearing test. Significant changes were observed on behavior tests after 5 weeks of treatment (p< 0.01) for the three test assayed. Latency in the behavior during the bar and grid test do correlate with catalepsy. Rigidity was tested with the rearing test. Physiological symptoms such as rigidity and immobility did appear after 5 weeks of treatment. An accurate experimental model of PD should reproduce the slow, progressive, and selective nigrostriatal dopaminergic degeneration seen in the disease. The lack of mortalitity in the treated group supports a good selection in the dose of rotenone applied. Although nigrostriatal degeneration can be confirmed by the specific staining, the behavioral results strongly suggest that animals developed PD