The absence of the potassium channel KCNQ4 affects the visual function

MARCELA VERA; SOFÍA STUPNIKI; BRUERA, MANUEL G.; MARÍA CONSTANZA PAZ; LEONARDO DIONISIO; ALAN VATER; PAULA SCHAIQUEVICH; CONTIN, MARÍA A; GUILLERMO SPITZMAUL

Buenos Aires

Congreso; SAN2022 Meeting; 2022

Sociedad Argentina de Investigación en Neurociencias

Voltage-gated KCNQ potassium channel subunits are responsible for the M-current that regulates neuronal excitability. We foundexpression of KCNQ4 in the retinal pigmented epithelium (RPE) and in the ciliary body (CB) of mouse eyes, suggesting that it couldparticipate in visual processing and aqueous humor formation. Using Kcnq4 knockout (KO) and wild-type (WT) mice we studied therole of KCNQ4 in vision. First, we analyzed Kcnq gene expression by qPCR. We found that in KO mice, the expression of Kcnq3 and -5 inthe RPE/retina did not change. On the other hand, in CB the expression of Kcnq3 increased 2.5-fold while the expression of Kcnq5decreased 30%. Then, we tested light perception by testing the innate aversion of rodents to it. We did not fi nd any differencesbetween the KO and WT in the test performance. To analyze the function of the neuronal visual pathway, we recordedelectroretinogram (ERG) in both genotypes. We observed no differences in a- and b-wave peaks and latency times between WT and KOin young animals, whereas in 50 weeks-old mice we observed a reduction trend in b-wave peak in KO while a-wave showed nodifferences. We also measured intraocular pressure (IOP) in young animals to evaluate CB function. We found a slight increase in theIOP in KO mice (13.6±1.0 to 15.8±1.1 mm Hg). In conclusion, the presence of the KCNQ4 subunit is necessary for the properexpression of the other Kcnq subunits and it would contribute to CB and retinal function.