Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells byPhotodynamic Therapy: an in vitro comparative study

FABIOLA N. VELAZQUEZ; MIRETTI, MARIANA; MARIA T. BAUMGARTNER; BEATRIZ L. CAPUTTO; TOMAS C.TEMPESTI; CESAR G. PRUCCA

Scientific Reports

springer Nature

Lugar: Berlin; Año: 2019 vol. 9 p. 1 - 1

2045-2322

lioblastoma multiforme is considered to be one of the most aggressive types of tumors of the central nervous system, with a poor prognosis and short survival periods of ~ one year. The current protocol for glioblastoma treatment includes the surgical excision of the primary tumor followed by radio and chemotherapy. Photodynamic therapy (PDT) is considered a promising strategy for the treatment of several types of tumors. Phthalocyanines (Pcs) are good photosensitizers (PSs) for PDT because they induce cell death in several cellular models. ZnPc is a well-known Pc, extensively tested in different cells and tumor models, but its evaluation on a glioblastoma model has been poorly studied. Herein, we compare the capacity of ZnPc and one of its derivatives, Zn(II)tetraminephthalocyanine (TAZnPc), to photoinactivate glioblastoma cells (T98G, MO59, LN229 and U87-MG) in culture. We measured the cellular uptake, the toxicity in the dark and the subcellular localization of the different Pcs, as