Photophysical and photodynamic analysis of different formulations of riboflavin

OSABA, MATIAS; CRISTIAN TEMPESTI, TOMAS; EDUARDO REVIGLIO, VICTOR

Medical hypothesis, discovery & innovation in optometry

International Virtual Ophthalmic Research Center

Lugar: Texas; Año: 2023 vol. 4 p. 181 - 181

ackground: Riboflavin (Rb) has been used in the ophthalmological procedure known as corneal cross-linking (CXL). Pathologies requiring this treatment include keratoconus, corneal ectasia, and infectious keratitis. Rb is instilled via different molecules that are transported into the tissues. However, each vehicle imparts different properties that alter the photodynamic behavior of Rb, leading to variable concentrations of free radicals within the medium. The objective of this study was to measure the concentrations of free radicals produced by commonly used Rb formulations. To determine the free radical production level of each formulation, L-tryptophan (L-Tryp)was used as a model substrate because it can be efficiently photo-oxidized. Methods: We investigated the photodegradation of L-Tryp and its kinetics upon light exposure. The spectra were recorded using a Shimadzu UV-1800 PC spectrophot