Photodynamic inactivation of T98g glioblastoma cells using ZnPc derivates.

CÉSAR G. PRUCCA; FABIOLA N. VELAZQUEZ; MARIANA MIRETTI; MARÍA T. BAUMGARTNER; TOMAS TEMPESTI ; BEATRIZ L. CAPUTTO

Mar del Plata

Congreso; LI Reunión SAIB 2015; 2015

SAIB

The development of novel therapeutic strategies for the treatment of tumors of the CNS is highly important. Glioblastomas are very aggressive brain tumors with poor prognostic and a very short survival period of approximately 1 year. The standard protocol for the treatment of these tumors include reductive surgery followed by radio and chemotherapy. Photodynamic therapy (PDT) has been studied for decades and several photosensitizers (PS) were synthetized and shown to be effective, in combination with light, to induce cell death to different tumor cells. Pthalocyanines are molecules with good properties as PS for PDT. The aim of this work was to evaluate the properties of Zinc pthalocyanines (ZnPc) and the derivates TAZnPc and TMAZnPc as PS for PDT in glioma cells (T98G glioblastoma cells). All the PS were innocuous in absence of light at concentrations menores o igual a 0,5 µM. However, after irradiation, both ZnPc and TAZnPc induce cell death in a concentration and light dose dependent manner. Both PS rapidly induces phosphatidylserine surface exposure, mitochondrial membrane depolarization, DNA fragmentation and activation of caspase -3, hallmarks of apoptosis. Nevertheless, ZnPc induces apoptosis with lower doses of light in comparison to TAZnPc. These results suggest that both, ZnPc and TAZnPc are good PS to continue in vivo studies in a Glioblastoma animal model.