InAlzheimer´s disease, where there is a decrease in acetylcholine levels in theCentral Nervous System affecting memory and learning, acetylcholinesterase(AChE) inhibitor drugs treatments are mainly used. In order to search newinhibitors, we studied A. communis, having previously demonstrated theeffect of its hydroalcoholic extract (ACHA) on AChE. For this species, it waspreviously detected quercetin (Q), rutin (R) and gallic acid (GA) in a 95%ethanolic extract. The aim of this work was to evaluated different ACHAfractions activity. They were obtained by partition of ACHA (dry, redissolvedin water) with CH₂Cl₂ and subsequent separation by columnchromatography (CC), with silica gel as the stationary phase and a gradient of increasingpolarity solvents as the mobile phase: 100% Hexane→ 100% CHCl₃→100% AcOEt→100%EtOH. 13 fractions were obtained: from G1 to G13. Then, the inhibition on AChEof each fraction was evaluated, according to the Ellman method, at a 250μg/mLconcentration. Chemical content of these fractions was analyzed in turn byHPLC-MS. Regarding AChE inhibition, G6 exhibited the highest activity: 72.13%,while G1 exerted the lowest: 5.83%. Remaining fractions inhibition were G3:38.72%; G7: 42.13%; G8:35.42%; G10: 46.38% and G11: 35.42%. HPLC-MS analysis resultsindicate that Q is present in G1 in low concentration; in G3, an unknowncompound of M+= 596.19 was observed. In G6, Q, R and AG were no detected, andin G7, G8, G10 and G11, same M+ to that of R compound was detected, but havingdifferent fragmentation pattern, which would indicate that it could be anothermetabolite. AG was not detected in any of the fractions. Relating thephytochemical composition to the observed inhibitory effect, it can beconcluded that this is not due to AG or Q, since they are not present in thesamples that showed greater inhibition, and in those that gave moderateinhibition a compound of the same M+ as R was detected. Further, the G3 componentand those present in the remaining fractions will be analyzed to predict,through the study of this species metabolomics, using multivariate analysis tools,which compound is responsible for the inhibitory activity in the most activesamples.