Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes

THEUMER, MG; CLOP, PD; RUBINSTEIN, HR; PERILLO, MA

AMER CHEMICAL SOC

Lugar: Washington; Año: 2012 vol. 116 p. 14216 - 14216

he toxicity of FB1 is usually explained through the enzymatic disruption of lipidic metabolism. However, it may lie in the thermodynamics of the membrane and its cooperative phase behavior rather than in the activity of individual proteins. Here, we investigate the effects FB1 at the molecular and mesoscopic levels in FB1-phospholipid mixed Langmuir films. Mean molecular area vs. FB1 molar fractions (xFB1) and phase diagram analysis allowed defining miscibility conditions and phase states at different xFB1. Surface potential measurements, evaluated as a function of the molecular packing and xFB1, revealed the FB1-induced change in the collective dipolar reorientation leaded to neutralization of charged films. Size, shape and distribution of 2D-domain analysis from epifluorescence data suggested the increase in the mixing entropy and film relaxation rate. Finally, PM-IRRAS revealed the orientation of FB1 with the amine-end (zwitterionic and negatively charged monolayers) or the tricarb