Diazinon toxicity in hepatic and spleen mononuclear cells is associated to early induction of oxidative stress

GIRÓN-PÉREZ, MANUEL IVÁN; MARY, VERÓNICA S.; RUBINSTEIN, HÉCTOR R.; TOLEDO-IBARRA, GLADYS A.; THEUMER, MARTÍN G.

INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH

TAYLOR & FRANCIS LTD

Año: 2021

0960-3123

iazinon is an organophosphorus pesticide, which may have potential toxic effects on the liver and immune system; however, the underlying mechanisms remain mostly unidentified. This work is aimed at evaluating the oxidative stress and cell cycle alterations elicited by low-dose diazinon in a rat liver cell line (BRL-3A) and spleen mononuclear cells (SMC) from Wistar rats. Diazinon (10?50 μM) caused early reactive oxygen species (ROS) generation (from 4 h) as well as increased O2?- level (from 0.5 h), which led to subsequent lipid peroxidation at 24 h, in BRL-3A cells. In SMC, diazinon (20 μM) produced similar increases in ROS levels, at 4 and 24 h, with the highest O2?- level being found at 4 h. Low-dose diazinon induced G1-phase arrest and cell death in hepatic cells and SMC. Therefore, diazinon could affect the liver and the immunological system through the premature oxidative stress induction. Abbreviations: O2?-: superoxide anion radical; ROS: reactive oxygen species; SMC