The lipid-mediated hypothesis of fumonisin B1 toxicodynamics tested in model membranes.

THEUMER, MG; CLOP, EM; RUBINSTEIN, HR; PERILLO, MA

Elsevier

Año: 2008 vol. 64 p. 22 - 22

p class="abstract">The disruption of lipidic metabolism was considered a good candidate to explain FB1 toxicity mechanism. In the present work we investigated molecular organizational changes induced by FB1-biomembrane interaction possibly involved in mycotoxic effects. FB1 was self-aggregated with a critical micellar concentration of 1.97 mM. FB1 (0-81.4 microM), decreased in a dose-dependent manner, the fluorescence anisotropy of TMA-DPH (from 0.349+/-0.003 to 0.1720+/-0.0035) in dpPC bilayers, whilst no differences were registered with DPH. At 5.6 microM in the subphase, FB1 increased the lateral surface pressure (pi) of a Langmuir film to an extent that depended on the monolayer composition (Deltapi dpPC:DOTAP 3:1>Deltapi dpPC:dpPA3:1>Deltapi dpPC), the molecular packing (Deltapi decreased linearly as a function of the initial pi) and the subphase pH (Deltapi pH 2.6>Deltapi pH 7.4 and maximal pi allowing the drug penetration pi cut-off was 34.3 and 27.7 mN/m at pH 2.63 an