Genotoxicity of aflatoxins and their precursors in human cells

THEUMER, M.G.; HENNEB, Y.; KHOURY, L.; SNINI, S.P.; TADRIST, S.; CANLET, C.; PUEL, O.; OSWALD, I.P.; AUDEBERT, M.

TOXICOLOGY LETTERS

ELSEVIER IRELAND LTD

Año: 2018 vol. 287 p. 100 - 100

0378-4274

flatoxins are found as food contaminant and some of them demonstrate a carcinogenic effect. The aflatoxins biosynthetic pathway involves 15 successive steps. The aim of this study was to compare the toxicity of aflatoxins and their precursors in three human cell lines. We tested the four aflatoxins and two of their metabolites; three early metabolic precursors and two late biosynthetic precursors. Cyclopiazonic acid, synthesized in parallel with aflatoxins, was also tested. The cytotoxicity and the genotoxicity was evaluated with the γH2AX assay in three human cell lines with different bioactivation capacities. Our results indicated that the most genotoxic chemicals in the three cell lines were in decreasing order sterigmatocystin (ST), aflatoxin B1 (AFB1), aflatoxicol (AFL), aflatoxin G1 (AFG1) and versicolorin A (VERA). Aflatoxin M1 (AFM1) demonstrated genotoxic property in only one cell line. The other tested compounds did not demonstrate any genotoxic activity. Overall, our r