Development of a bioelectrode fabricated with a multilayer thin film of poly(diallyldimethylammonium)/gold-nanoparticle/lactate oxidase for analysis of L-lactate in food samples

O. E. LINAREZ PÉREZ

SENSORS AND ACTUATORS B-CHEMICAL

ELSEVIER SCIENCE SA

Lugar: Amsterdam; Año: 2017 vol. 247 p. 830 - 830

0925-4005

ultilayer films made of poly(diallyldimethylammonium) (PDDA),gold nanoparticles (AuNP) of 10 nm diameter and lactate oxidase (LOx),were built for the first time, by layer-by-layer (LBL) assembly techniqueonto both quartz and gold substrates. The surface plasmon resonance (SPR)band of AuNP was used for monitoring the film growth and adsorptionkinetics on PDDA monolayer supported onto quartz substrate. In the caseof polycrystalline gold surfaces, electrochemical characterization wasperformed by means of cyclic voltammetry (CV) and electrochemicalimpedance spectroscopy (EIS). The latter system was used as bioelectrodefor L-lactate quantification, showing short time response (