AMBROGGIO ERNESTO
Artículos
Título:
Detecting phospholipase activity with the amphipathic lipid packing sensor motif of ArfGAP1
Autor/es:
QUARTINO, PABLO YUNES; FIDELIO, GERARDO DANIEL; MANNEVILLE, JEAN-BAPTISTE; GOUD, BRUNO; AMBROGGIO, ERNESTO ESTEBAN
Revista:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Año: 2018
ISSN:
0006-291X
Resumen:
he amphipathic lipid packing sensor (ALPS) motif of ArfGAP1 brings this GTPase activating protein to membranes of high curvature. Phospholipases are phospholipid-hydrolyzing enzymes that generate different lipid products that alter the lateral organization of membranes. Here, we evaluate by fluorescence microscopy how in-situ changes of membrane lipid composition driven by the activity of different phospholipases promotes the binding of ALPS. We show that the activity of phospholipase A2, phospholipase C and phospholipase D drastically enhances the binding of ALPS to the weakly-curved membrane of giant liposomes. Our results suggest that the enzymatic activity of phospholipases can modulate the ArfGAP1-mediated intracellular traffic and that amphiphilic peptides such as the ALPS motif can be used to study lipolytic activities at lipid membranes