A closer look into the physical interactions between lipid membranes and layered double hydroxide nanoparticles

VASTI, CECILIA; AMBROGGIO, ERNESTO; ROJAS, RICARDO; GIACOMELLI, CARLA E.

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Año: 2020 vol. 191

0927-7765

ayered double hydroxide nanoparticles (LDH-NPs) constitute promising nanocarriers for drug and gene delivery. Although their cell internalization has been studied, the interaction between LDH-NPs and biological membrane models, such as giant unilamellar vesicles (GUVs), remains unexplored. These vesicles are widely-used membrane models that allow minimizing the complexity and uncertainty associated with biological systems to study the physical interactions in the absence of cell metabolism effects. With such an approach the physicochemical properties of the membrane can be differentiated from the biological functionalities involved in cell internalization and the membrane-mediated internalization can be directly understood. In this work, we describe for the first time the interaction of LDH-NPs with freestanding negatively charged POPC:POPS GUVs by fluorescence microscopy. The experiments were performed with fluorescein labeled LDH-NPs of about 100 nm together with different fluoropho