AMBROGGIO ERNESTO
Capítulos de libros
Título:
Piercing the lipid bilayers with peptides.
Autor/es:
AMBROGGIO ERNESTO E.; BAGATOLLI LUIS; GOORMAGHTIGH E.; FOMINAYA J.; GASSET MARIA
Libro:
Advances in Planar Lipid bilayers and Liposomes (Vol. 5)
Referencias:
Año: 2005; p. 1 - 44
Resumen:

Disruption of the permeability barrier of biological membranes is one of the

mechanisms exploited by nature to allow the physiological or pathological chemical

communication between otherwise isolated-like compartments. Despite the molecular

complexity of these membranes, the lipid bilayer backbone is the permeability guardian

and therefore the universal target of numerous peptides and small proteins that act as

perfect weapons. This pathway of cell life interference is that primarily used by the

peptides forming part of the innate host defence against pathogens, some of which are

exploited as antibiotics in ectopic infection treatments. Similarly, the phage holins,

naturally tailored for allowing the release of the phage progeny at the last stage of the

lytic cycle, have their application in biotechnological contention and in dairy industry.

The necessity to modify and regulate the genome and transcriptome of living organism

has demanded the access to cell interior without damage. The knowledge accumulated

from the peptide-membrane interaction field has allowed the rational ?de novo? design

of novel peptide sequences that either alone, in combination or in tandem incorporate

the capacity to produce the non-toxic membrane permeation events required for

efficient gene transfer. RAWA, a 23 amino acid peptide, serves as a paradigm of this

synthetic approach.

KEYWORDS: membrane permeation, DNA condensation, amphipatic helical peptides,

non-viral gene carriers, transfection.

non-viral gene carriers, transfection.

non-viral gene carriers, transfection.

membrane permeation, DNA condensation, amphipatic helical peptides,

non-viral gene carriers, transfection.