AMBROGGIO ERNESTO
Congresos y reuniones científicas
Título:
Lipid-packing and membrane curvature for the regulation of protein-membrane interaction of the Arf1/ArfGAP1-mediated trafficking
Autor/es:
AMBROGGIO ERNESTO ESTEBAN; JEAN-BAPTISTE MANNEVILLE; BRUNO GOUD; BRUNO ANTONNY
Lugar:
Puerto Natales
Reunión:
Workshop; EMBO Workshop: Current advances in membrane trafficking: Implications for polarity and diseases; 2014
Institución organizadora:
EMBO
Resumen:
The recruitment of coatomer (COPI) by the small G-protein Arf1 (GTP-bound form) to the Golgi
generates highly-curved COPI-coated membrane buds, which give rise to transport vesicles. The
GTPase activating protein for Arf1, ArfGAP1, has been shown to preferentially interact with highly
curved membranes where it promotes fast GTP hydrolysis on Arf1. This adsorption to highly
curved membranes is due to the presence of two adjacent lipid-packing sensor motifs, called
ALPS 1 and ALPS 2, in the middle of the ArfGAP1 sequence. Here I will introduce two cases
of study: 1-how lipid packing and membrane curvature impacts on the association and activity of
ArfGAP1when GTP-Arf1 is associated to low and high-curvature membranes; and 2-how membrane
curvature is important and necessary for the recruitment of Arfaptin2, induced by Arf1 activation, to
the surface of liposomes and lipid tubes.