The effect of lipid packing and membrane curvature on protein binding and traffic

AMBROGGIO ERNESTO E.

Puerto Iguazú

Congreso; 56th International Conference on the Bioscience of Lipids; 2015

International Conference on the Bioscience of Lipids

The understanding of how membrane physics control biological processes in cells is a key step to better comprehend biology. One aspect is how lipid packing and membrane curvature dictates the rules for lipid/protein interactions and/or protein sorting. Here I will introduce different cases of how this physical principle modulates, or not, the association and trafficking of proteins: 1-how lipid packing and membrane curvature impacts on the association and activity of ArfGAP1when GTP-Arf1 is associated to low and high-curvature membranes; and 2-in what way membrane curvature is important and necessary for the recruitment of Arfaptin2, induced by Arf1 activation, to the surface of liposomes and lipid tubes; 3-how in-situ phospholipase activity induces lipid-packing defects that are "sensed" by ALPS, the amphipatic lipid packing sensor motif of ArfGAP1; and 4-is membrane curvature responsible for trans-membrane domain sorting at the Golgi apparatus?