Identification, Localization, and Quantification of Neuronal Cell Membrane Receptors with Plasmonic Probes: Role of Protein Kinase D1 in Their Distribution

JUAN C. FRAIRE; M. LUJAN MASSERONI ; IGNACIO JAUSORO; EDUARDO M. PERASSI; ALBERTO M. DIAZ AÑEL; EDUARDO A. CORONADO

ACS NANO

AMER CHEMICAL SOC

Lugar: Washington D. C., Estados Unidos; Año: 2014 vol. 8 p. 8942 - 8942

1936-0851

etecting, imaging, and being able to localize the distribution of several cell membrane receptors on a single neuron are very important topics in neuroscience research. In the present work, the distribution of metabotropic glutamate receptor 1a (mGluR1a) density on neuron cells on subcellular length scales is determined by evaluating the role played by protein kinase D1 (PKD1) in the trafficking of membrane proteins, comparing the distribution of mGluR1a in experiments performed in endogenous PKD1 expression with those in the presence of kinase-inactive protein kinase D1 (PKD1-kd). The localization, distribution, and density of cell surface mGluR1a were evaluated using 90 nm diameter Au nanoparticle (NP) probes specifically functionalized with a high-affinity and multivalent labeling function, which allows not only imaging NPs where this receptor is present but also quantifying by optical means the NP density. This is so because the NP generates a density (