Cow's milk stimulated lymphocyte proliferation and TNFalpha secretion in hypersensitivity to cow's milk protein.

MOTRICH RD; GOTTERO C; REZZONICO C II; REZZONICO C; RIERA CM; RIVERO V

CLINICAL IMMUNOLOGY

Elsevier

Lugar: Los Angeles; Año: 2003 vol. 109 p. 203 - 203

1521-6616

p class="abstract">Although there is no reliable single laboratory test available for the diagnosis of cow´s milk allergy, if an allergic mechanism is suspected, a number of laboratory studies may be useful in delineating specific proteins responsible for these disorders. In the current study we analyzed in vitro lymphocyte proliferation assays, specific secretion of TNFalpha in supernatant cultures and specific IgE, IgG, and IgA in a group of patients with hypersensitivity to cow´s milk antigens. The stimulation index against a cow´s milk antigen mixture, alpha-lactalbumin, beta-lactoglobulin, and casein was significant higher in the group of patients maintained on cow´s milk-free diet for less than 4 months compared with the values observed in the control group and in the group of patients without a close contact to cow´s milk proteins. A significant increase in TNF-alpha secretion was observed in supernatants from patients with close contact to cow´s milk (CM). Specific IgE was det