Enzyme-free Immunoassay Using Silver Nanoparticles for Detection of Gliadin at ultra-low concentrations

MERCADAL, PABLO; FRAIRE, JUAN C.; MOTRICH, RUBEN D.; CORONADO, EDUARDO A.

ACS Omega

American Chemistry Society

Lugar: Estocolmo; Año: 2018

0976-3961

he combination of the optical properties of silver nanoparticles with some of the tool-kits of the widespread used ELISA method for antigen quantification immunoassays, give rise to an enzyme free, low cost, fast and more sensitive optical method denoted as Intensity Depletion Immuno-linked Assay (IDILA), that can be performed directly in colloidal dispersions without any immobilization of the capture antibody, antigen, secondary and primary antibodies on a substrate. The capabilities of the method for quantifying antigens at ultralow concentrations in colloidal dispersions as well as its performance are demonstrated for specific antigens of importance in medicine and in food science. Copyright © 2018 VBRI Press.