Differences in T regulatory cells between mouse strains frequently used in immunological research

GODOY, GLORIA J.; PAIRA, DANIELA A.; OLIVERA, CAROLINA; BRESER, MARIA L.; SANCHEZ, LEONARDO R.; MOTRICH, RUBEN D.; RIVERO, VIRGINIA E.

IMMUNOLOGY LETTERS

ELSEVIER SCIENCE BV

Año: 2020 vol. 223 p. 17 - 17

0165-2478

oxp3+ Regulatory T cells (Tregs) are essential for the maintenance of tolerance to self. Therefore, it is expected that lower numbers and/or less than optimal function could impact on the functioning of the immune system, and thereby contributing to the development of autoimmune diseases. In the present report, by comparing Tregs from most frequently used mouse strains in immunological research (C57BL/6 (B6), BALB/c and NOD), we provide evidence showing that the NOD mouse strain, highly predisposed to develop autoimmune responses, exhibit a generalized decreased in Tregs counts with enhanced proportions of CD44hiCD62Llow Tregs when compared with BALB/c mice. No major differences were observed in Helios+ or Helios- Tregs between strains. The expression of CXCR3, CCR5 and CCR6 on Tregs from all strains showed minor proportions of CXCR3+ and CCR5+ cells in NOD Tregs. Naïve CD4+CD25- T cells from NOD mice also showed decreased capacity to induce in vitro iTregs when compared with B6 and B