Ultraluminescent Biophotonics assays for In Flow Escherichia Coli bacteria detection and counting towards perspectives on Single Biostructure analysis at molecular level

LUNA R. GOMEZ-PALACIOS; CARINA SALINAS; VALERIA AME; A. GUILLERMO BRACAMONTE

Rome

Congreso; International Conference and Expo on Clinical Microbiology; 2022

In this presentation it is showed Research and Development related with the design and synthesis of Ultraluminescent Nano-emitters based on Metal Enhanced Fluorescence (MEF) phenomena. It is showed gold Core-shell Nanoparticles with silica spacers and modified Fluorescent Silica shells with Enhanced and stable Luminescent properties. These Nano-emitters were used as Nanolabellers for Escherichia Coli bacteria. In this manner it was achieved Bright and Ultraluminescent Nano-Biostructures that afforded to faster detection within low bacteria concentrations at the level of single bacterium detection by Laser Fluorescence Microscopy. In this context it is highlighted and discussed the importance of the Optical properties of Nano-labellers and Biostructures for targeted applications from the Nano-Biostructure formation. Thus, it is showed In Flow Cytometry detection of Nano-Biostructures at varied levels of concentrations. In addition, it is discussed perspectives about how it could be managed these Hybrid Nano-Biostructures to record additional information from single Biostructure analysis. In this manner it is leaded to new potential approaches within Biophotonics by Targeted Light Delivery through Biostructures that just not improve only Biodetection, but as well it increases the power of analysis towards Single Cells applications.