In Flow Escherichia Coli bacteria detection: from single Metal Enhanced Fluorescence Nano-Biostructures towards higher sized Ultraluminescent assemblies

LUNA R. GOMEZ-PALACIOS; CARINA SALINAS; VALERIA AME; A. GUILLERMO BRACAMONTE

Buenos Aires

Workshop; Workshop: Frontiers in Nanobiotechnology III; 2022

Facultad de Farmacia y Bioquímica de la UBA Buenos Aires

In this presentation it is showed Research and Development related with the design and synthesis of Ultraluminescent Nano-emitters based on Metal Enhanced Fluorescence (MEF) phenomena. It is showed gold Core-shell Nanoparticles with silica spacers and modified Fluorescent Silica shells with Enhanced and stable Luminescent properties. These Nano-emitters were used as Nanolabellers for Escherichia Coli bacteria. In this manner it was achieved Bright and Ultraluminescent Nano-Biostructures that afforded to faster detection within low bacteria concentrations at the level of single bacterium detection by Laser Fluorescence Microscopy. In this context it is highlighted and discussed the importance of the Optical properties of Nano-labellers and Biostructures for targeted applications from the Nano-Biostructure formation. Thus, it is showed Laser Fluorescence Microscopy imaging, and In Flow Cytometry detection of Nano-Biostructures at varied levels of concentration. Moreover, it was evaluated In Flow Bio-assays with potential applications to record additional Biophotonic information by Targeted Light Delivery through Biostructures.