Potential-Assisted Adsorption of Bovine Serum Albumin onto Optically-Transparent Carbon Electrodes

TOMÁS E. BENAVIDEZ; CARLOS D. GARCIA

AMER CHEMICAL SOC

Lugar: Washington; Año: 2013 p. 14154 - 14154

BSTRACT This manuscript describes the effect of the applied potential on the adsorption of bovine serum albumin (BSA) to optically transparent carbon electrodes (OTCE). To decouple the effect of the applied potential from the high affinity of the protein for the bare surface, the surface of the OTCE was initially saturated with a layer of BSA. Experiments described in the manuscript show that potential values higher than +500 mV induced a secondary adsorption process (not observed at open-circuit potentials), yielding significant changes in the thickness (and adsorbed amount) of the BSA layer obtained. Although the process showed a significant dependence on the experimental conditions selected, the application of higher potentials, selection of pH values around the isoelectric point (IEP) of the protein, high concentrations of protein, and low ionic strengths yielded faster kinetics and the accumulation of larger amounts of protein on the substrate. These experiments, obtained around