Adsorption of Soft and Hard Proteins onto OTCEs under the Influence of an External Electric Field

TOMÁS E. BENAVIDEZ; DANIEL TORRENTE; MARCELO MARUCHO; CARLOS D. GARCIA

AMER CHEMICAL SOC

Lugar: Washington; Año: 2015 vol. 31 p. 2455 - 2455

he adsorption behavior of hard and soft proteins under the effect of an external electric field was investigated by a combination of spectroscopic ellipsometry and molecular dynamics (MD) simulations. Optically transparent carbon electrodes (OTCE) were used as conductive, sorbent substrates. Lysozyme (LSZ) and ribonuclease A (RNase A) were selected as representative hard proteins, whereas myoglobin (Mb), α- lactalbumin (α-LAC), bovine serum albumin (BSA), glucose oxidase (GOx), and immunoglobulin G (IgG) were selected to represent soft proteins. In line with recent publications from our group, the experimental results revealed that while the adsorption of all investigated proteins can be enhanced by the potential applied to the electrode, the effect is more pronounced for hard proteins. In contrast with the incomplete monolayers formed at open-circuit potential, the application of +800 mV to the sorbent surface induced the formation of multiple layers of protein. These resul