Electrochemically-Preadsorbed Collagen Promotes Adult Human Mesenchymal Stem Cell Adhesion

TOMÁS E. BENAVIDEZ; MARISSA E. WECHSLER; MADELEINE M. FARRER; RENA BIZIOS; CARLOS D. GARCIA

Tissue Engineering Part C: Methods

Mary Ann Liebert, Inc.

Lugar: New Rochelle, NY 10801; Año: 2016 p. 69 - 69

2152-4947

he present article reports on the effect of electric potential on the adsorption of collagen type I (the most abundant component of the organic phase of bone) onto optically transparent carbon films (OTCE) and its mediation on subsequent adhesion of adult, human, mesenchymal stem cells (hMSCs). For this purpose, adsorption of collagen type I was investigated as a function of the protein concentration (0.01, 0.1, and 0.25 mg/mL) and applied potential (open circuit potential (OCP; control), +400, +800, and +1500 mV). The resulting substrate surfaces were characterized using spectroscopic ellipsometry (SE), atomic force microscopy (AFM), and cyclic voltammetry (CV). Adsorption of collagen type I onto OTCE was affected by the potential applied to the sorbent surface and the concentration of protein. The higher the applied potential and protein concentration, the higher the adsorbed amount (collagen). It was also observed that the application of potential values higher than +800 mV