Supramolecular Architecture Based on the Self-Assembling of Multiwall Carbon Nanotubes Dispersed in Polyhistidine and Glucose Oxidase: Characterization and Analytical Applications for Glucose Biosensing.

PABLO R. DALMASSO, MARÍA L. PEDANO, GUSTAVO A. RIVAS

ELSEVIER ADVANCED TECHNOLOGY

Lugar: Amsterdam; Año: 2013 vol. 39 p. 76 - 76

e report for the first time the development of a sensitive and selective glucose biosensor based on the self-assembling of multiwall carbon nanotubes (MWCNTs) dispersed in polyhistidine (Polyhis) and glucose oxidase (GOx) on glassy carbon electrodes (GCE). The supramolecular architecture was characterized by SEM, FT-IR and electrochemical techniques. The optimum multistructure was obtained with five (MWCNT-Polyhis/GOx) bilayers and one layer of Nafion as anti-interferent barrier. The sensitivity at 0.700 V was (1.94±0.03)mA M−1 (r=0.9991), with a linear range between 0.25 and 5.00 mM, a detection limit of 2.2 μM and a quantification limit of 6.7 μM with minimum interference from lactose (1.5%), maltose (5.7%), galactose (1.2%), ascorbic acid (1.0%), and uric acid (3.3%). The biocatalytic layer demonstrated to be highly reproducible since the R.S.D. for 10 successive amperometric calibrations using the same surface was 3.6%. The sensitivity of