Interaction of Green Silver Nanoparticles with Model Membranes: Possible Role in the Antibacterial Activity.

ANIKE P.V. FERREYRA MAILLARD; PABLO R. DALMASSO*; BEATRIZ A. LÓPEZ DE MISHIMA; AXEL HOLLMANN*

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2018 vol. 171 p. 320 - 320

0927-7765

ilver nanoparticles (AgNPs) constitute a very promising approach for overcoming the emergence of antibiotic resistance bacteria. Although their mode of action could be related with membrane damage, the AgNPs-lipid membrane interaction is still unclear. In this sense, the present work investigated the interaction of model lipid membranes with AgNPs coated with different capping agents such as citrate (C-AgNPs) and phytomolecules (G-AgNPs) obtained via a green synthesis. The AgNPs-membrane interactions were evaluated studying i) the surface pressure changes on both zwitterionic (DMPC) and negatively charged (DMPC:DMPG) lipid monolayers, ii) the zeta potential and DLS of DMPC:DMPG liposomes and iii) Zeta potential on Escherichia coli membranes, incubated with this nanomaterials. The results showed that both negatively charged-AgNPs can interact with these lipid monolayers inducing an increase in the surface pressure but G-AgNPs presented a significantly higher affinity toward both monola