New Trends in the Development of Electrochemical Biosensors for the Quantification of microRNAs.

MICHAEL LÓPEZ MUJICA, PABLO A. GALLAY, FABRIZIO PERRACHIONE, ANTONELLA E. MONTEMERLO; LUIS A. TAMBORELLI, VIRGINIA M. VASCHETTI; DAIANA F. REARTES; SOLEDAD BOLLO; MARCELA C. RODRÍGUEZ; PABLO R. DALMASSO; MARÍA D. RUBIANES, GUSTAVO A. RIVAS

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

ELSEVIER SCIENCE BV

Año: 2020 vol. 189

0731-7085

icroRNAs (miRNAs) are non-coding regulatory RNAs that play an important role in RNA silencingand post-transcriptional gene expression regulation. Since their dysregulation has been associated with Alzheimer disease, cardiovascular diseases and different types of cancer, among others, miRNAs can be used as biomarkers for early diagnosis and prognosis of these diseases. The methods commonly used to quantify miRNAs are, in general, complex, costly, with limited application for point-of-care devices or resource-limited facilities. Electrochemical biosensors, mainly those based on nanomaterials, have emerged as a promising alternative to the conventional miRNA detection methods and have paved the way to the development of sensitive, fast, and low-cost detection systems. This review is focused on the most relevant contributions performed in the field of electrochemical miRNAs biosensors between 2017 and the beginning of 2020. The main contribution of this article is the critical discussion