Two Birds with One Stone: Integrating Exfoliation and Immunoaffinity Properties in Multi-walled Carbon Nanotubes by Non-Covalent Functionalization with Human Immunoglobulin G.

MICHAEL LÓPEZ MUJICA; ALEJANDRO TAMBORELLI; VIRGINIA M. VASCHETTI; L. CAROLINA ESPINOZA, SOLEDAD BOLLO; PABLO R. DALMASSO*; GUSTAVO A. RIVAS*

MIKROCHIMICA ACTA

SPRINGER WIEN

Año: 2023 vol. 190

0026-3672

n innovative strategy is proposed to simultaneously exfoliate multi-walled carbon nanotubes (MWCNTs) and generate MWCNTs with immunoafnity properties. This strategy was based on the non-covalent functionalization of MWCNTs with human immunoglobulin G (IgG) by sonicating 2.5 mg mL−1 MWCNTs in 2.0 mg mL−1 IgG for 15 min with sonicator bath. Impedimetric experiments performed at glassy carbon electrodes (GCE) modifed with the resulting MWCNT-IgG nanohybrid in the presence of anti-human immunoglobulin G antibody (Anti-IgG) demonstrated that the immunoglobulin retains their biorecognition properties even after the treatment during the MWCNT functionalization. We proposed, as proofof-concept, two model electrochemical sensors, a voltammetric one for uric acid quantifcation by taking advantages of the exfoliated MWCNTs electroactivity (linear range, 5.0× 10−7 M - 5.0× 10−6 M; detection limit, 165 nM) and an impedimetric immunosensor for the detection of Anti-IgG throu