Mass spectrometry studies of lycodine-type Lycopodium alkaloids: sauroxine and N-demethylsauroxine

DIEGOA. CIFUENTE; MARIANA G. VALLEJO, ;ARÍA G. ORTEGA, JOSÉ L. CABRERA, VALERIA P. CARLINI, SUSANA RUBIALES DE; MARIA G. ORTEGA; JOSÉL. CABRERA; VÍCTOR S.MARTÍN; CARLOS E. TONN; ALICIA M. AGNESE; CARLOS E. ARDANAZ

JOHN WILEY & SONS LTD

Lugar: LOndres; Año: 2014 vol. 28 p. 2690 - 2690

ATIONALE: Sauroxine and N-demethylsauroxine are lycodine-type Lycopodium alkaloids. In recent years, Lycopodium alkaloids have gained significant interest due to their unique skeletal characteristics as well as due to their acetylcholinesterase activity. It is known that drugs that inhibit acetylcholinesterase can be used to treat the early stages of Alzheimer?s disease. METHODS: Sauroxine and N-demethylsauroxine were isolated from the aerial parts of Huperzia saururus (Lam.) Trevis. Electron ionization mass spectrometry (EI-MS) (low resolution) and collision-induced dissociation tandem mass spectrometry (CID-MS/MS) fragmentation was conducted using an ion trap, GCQ Plus mass spectrometer with MS/MS. Electron ionization high-resolution mass spectrometry (EI-HRMS) was performed in a magnetic sector mass spectrometer (Micromass VG). RESULTS: Using GC/EI-CID-MS/MS we obtained different fragmentation routes that connect all the ionic populations. In addition, the use of EI-HRMS