ULTRASONIC-ASSISTED EXTRACTION OF ANTHRAQUINONES FROM HETEROPHYLLAEAPUSTULATA HOOK F. (RUBIACEAE) USING ETHANOL?WATER MIXTURESM

M.F.BARRERA VÁZQUEZ; L.R. COMINI,; R.E. MARTINI; S.C.NÚÑEZ MONTOYA; S. BOTTINI; J.L. CABRERA.

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 p. 278 - 278

n this study, ultrasound-assisted extraction (UAE) was evaluated as an easier and more efficientalternative method for the isolation of anthraquinones (AQs) from Heterophyllaea pustulata Hook f.(Rubiaceae), compared with conventional extraction techniques. The extraction was performed usingan ethanol?water solution as solvent. The influence of different operating conditions, as temperature,time, solvent composition, and solvent/sample ratio and its relationship with ultrasonic techniques wasanalyzed. For this purpose and to achieve global optimization of the ultrasound-assisted extraction of H.pustulata, Taguchi experimental design with a L9 orthogonal array was applied.The results showed that the yield obtained with the ultrasound assisted extraction is approximatelytwice higher than the traditional Soxhlet technique, and Taguchi analysis demonstrated that the mostinfluential factor is solvent concentration, followed by time, temperature, and solvent/sample ratio. Thesolvent composit