ANTIFUNGAL ACTIVITY OF A PRENYLATED FLAVONOID FROM Dalea elegans AGAINST Candida albicans BIOFILMS

? MARIANA A PERALTA; MARÍA A DA SILVA; MARÍA G ORTEGA; JOSÉ L CABRERA; MARÍA G PARAJE.

ELSEVIER GMBH

Año: 2015 vol. 22 p. 975 - 975

ackground:ThecontinuingemergenceofinfectionswithantifungalresistantCandidastrainsrequiresaconstantsearchfornewantifungaldrugs,withtheplantkingdombeinganimportantsourceofchemicalstructures.Purpose:Thepresentstudyinvestigatedtheantifungaleffectof2,4-dihydroxy-5-(1,1-dimethylallyl)-8-prenylpinocembrin(8PP,formerly6PP),anaturalprenylflavonoid,onCandidaalbicansbiofilms,andcomparedthiswithanazoleantifungal(fluconazole)bystudyingthecellularstressandantioxidantresponse.Studydesign/methods:Thefluconazolesensitive(SCa)andazole-resistant(RCa)C.albicansstrainswereused,withbiofilmformationbeingstudiedusingcrystalviolet(CV)andconfocalscanninglasermicroscopy(CSLM).Theminimalinhibitoryconcentrationforsessilecells(SMIC)wasdefinedastheconcentrationofantifungalthatcauseda50%(SMIC50)and80%(SMIC80)reductionoftreatedbiofilms.Thereactiveoxygenspecies(ROS)weredetectedbythereductionofnitrobluetetrazolium(NBT),andreactivenitrogenintermediates(RNI)weredeterminedbytheGriessassay.Theactivitiesofthesuperoxidedismu