Interactions of a prenylated flavonoid from Dalea elegans with fluconazole against azole- resistant Candida albicans

SEBASTIÁN BARCELÓ; MARIANA PERALTA; MAXIMILIANO CALISE; SOLEDAD FINCK; GABRIELA ORTEGA; ROBERTO A. DIEZ; JOSÉ LUIS CABRERA; CRISTINA PÉREZ

PHYTOMEDICINE

ELSEVIER GMBH

Año: 2017 vol. 32 p. 24 - 24

0944-7113

ackground: The prenylated flavonoid 2′, 4′-dihydroxy-5′-(1′″, 1′″-dimethylallyl)-8-prenylpinocembrin (8PP,formerly 6PP) shows antifungal activity, inhibits rhodamine 6G efflux and reverses fluconazole (FCZ) resistancein azole-resistant Candida albicans overexpressing cdr1, cdr2 and mdr1 transporters.Purpose and design: In this paper, we tried to characterize 8PP in vitro interactions on the cell growth andlethality of C. albicans. We also initiated preliminary in vivo toxicological studies on mice.Methods: The effects of 8PP and FCZ on cell growth and viability of C. albicans were evaluated by CLSI guidelines.The checkerboard assay was used to search for interactions on cell growth. The time-kill assay was used tostudy fungicidal effects. Acute toxicity was evaluated at a single dose schedules.Results: From the checkerboard design, and using a starting inoculum of 103 CFU/ml, the fractional inhibitoryconcentration (FIC) of FCZ and 8PP could be