The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms

PERALTA, MARIANA ANDREA; ORTEGA, MARÍA GABRIELA; CABRERA, JOSÉ LUIS; PARAJE, MARÍA GABRIELA

FOOD AND CHEMICAL TOXICOLOGY

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2018 vol. 114 p. 285 - 285

0278-6915

bstractThe antioxidant effect of 8PP, a prenylflavonoid from Dalea elegans on Candidaalbicans biofilms, was investigated. We previously reported that sensitive (SCa) andresistant C. albicans (RCa) biofilms were strongly inhibited by this compound, in a dosedependingmanner (50 μM-100 μM), with a prooxidant effect leading to accumulation ofendogenous oxidative metabolites and increased antioxidant defenses. In this work, theantifungal activity of high concentrations of 8PP (200-1000 μM), the cellular stressimbalance and the architecture of biofilms were evaluated. Biofilms were studied by crystalviolet and confocal scanning laser microscopy (CSLM) with COMSTAT analysis.Superoxide anion radical, the activity of the superoxide dismutase and the total antioxidantcapacity were measured. Intracellular ROS were detected by a DCFH-DA and visualizedby CSLM; reactive nitrogen intermediates by Griess.An antioxidant effect was detected at 1000 μM and levels of oxidant metabolit