QUERCETIN 3, 7, 3´, 4´-TETRASULPHATE INHIBITS THE PLASMINOGEN ACTIVATOR INHIBITOR-1 BUT NOT THE TISSUE-TYPE PLASMINOGEN ACTIVATOR EXPRESSION IN HUMAN NORMAL FIBROBLASTS,

HUGO GUGLIELMONE; SUSANA C. ALICIA M. AGNESE; SUSANA C. NUÑEZ-MONTOYA; CLAUDIA G. PELLIZAS; MARCELO FUENTES; JOSÉ L. CABRERA ; ANA C. DONADIO

International Journal of Pharmacognosy and Phytochemistry

RECENT SCIENCE PUBLICATIONS

Lugar: Londres; Año: 2015 vol. 30 p. 1355 - 1355

2051-7858

BSTRACTSulphated flavonoids quercetin 3, 7, 3´, 4´-tetrasulphated (QTS) and quercetin 3-acetyl-7, 3´, 4´-trisulphate (ATS), obtained from Flaveria bidentis have demonstrated some antithrombotic properties. We analyzed whether both compounds affected the expression of tissue-plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1), the components of the fibrinolytic system. Normal human fibroblasts were pretreated with different concentrations of each sulphated flavonoid (0.1 μM to 500 μM), during 2 and 3 h followed by a 24 h incubation with phorbol myristate acetate (PMA). Results of the tPA and PAI-1 expression compared to control, showed different behaviors for the two flavonoids studied. A null inhibitory effect on the tPA expression was detected at all the concentrations for QTS and ATS. In contrast, QTS from 50 μM onwards showed a significant inhibitory effect on PAI-1 expression (p< 0.05). The profibrinolytic activity ofQTS enhances its antithromb