Protective effect of quercetin in gentamicin-induced oxidative stressin vitro and in vivo in blood cells. Effect on gentamicin antimicrobialactivity

PAMELA SOLEDAD BUSTOS; ROMINA DEZA-PONZIOD; PAULINA LAURA PÁEZ; INES ALBESA; JOSÉ LUIS CABRERA; MIRIAM BEATRIZ VIRGOLINI; MARÍA GABRIELA ORTEGA

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 48 p. 253 - 253

1382-6689

e have evaluated the effect of gentamicin and gentamicin plus quercetin on ROS production, endoge-nous antioxidant defenses (SOD and CAT) and lipid peroxidation in vitro on human leukocytes and in vivoon whole rat blood. Gentamicin generated ROS production in human leukocytes, produced a dual effecton both enzymes dosage-dependent and generated an increase in lipid peroxidation. Quercetin, in leuko-cytes stimulated by gentamicin, showed more inhibitory capacity in ROS production than the referenceinhibitor (vitamin C) in mononuclear cells and a similar protective behavior at this inhibitor in polymor-phonuclear cells. Quercetin, in both cellular systems, tend to level SOD and CAT activities, reaching basalvalues and could prevent lipidic peroxidation induced by gentamicin. The results in Wistar rats confirmedthat therapeutic doses of gentamicin can induce oxidative stress in whole blood and that the gentamicintreatment plus quercetin can suppress ROS generation, collaborate with SOD a