QUIROGA RODRIGO
Congresos y reuniones científicas
Título:
MOLECULAR AND FUNCTIONAL CHARACTERIZATION OF S-RNAses FROM A NATURAL POPULATION OF Nicotiana alata
Autor/es:
ROLDÁN, JA; QUIROGA, R; ROJAS, HJ; GOLDRAIJ, A
Lugar:
San Miguel de Tucumán, Tucumán , Argentina.
Reunión:
Congreso; XLV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Institución organizadora:
SAIB
Resumen:
We report here the molecular and functional characterization of
novel S-RNase candidates identified from a natural population of
Nicotiana alata. Using degenerated oligonucleotides based on
conserved domains of S-RNases, we identified by RT-PCR ten style
sequences homologous to S-RNases, including three previously
reported functional S-RNases and seven novel S-RNase candidates.
Six of these candidates showed, by allele-specific RT-PCR, plant-
specific expression in the natural population and style specific
amplification, which increased gradually during bud maturation,
consistent with the reported S-RNase expression. In contrast, the
S63 sequence was present in all plants examined and was
ubiquitously expressed in different organs and bud developmental
stages. Genetic analysis confirmed the association with self-
incompatible function in five S-RNase candidates while S5 and S63
appeared to be nonfunctional relic S-RNases. Phylogenetical
analysis showed the Nicotiana alata S-RNases were included in
eight transgeneric lines confirming that this species represents a
broad sample of allelic variation at the S-locus of the Solanaceae.
novel S-RNase candidates identified from a natural population of
Nicotiana alata. Using degenerated oligonucleotides based on
conserved domains of S-RNases, we identified by RT-PCR ten style
sequences homologous to S-RNases, including three previously
reported functional S-RNases and seven novel S-RNase candidates.
Six of these candidates showed, by allele-specific RT-PCR, plant-
specific expression in the natural population and style specific
amplification, which increased gradually during bud maturation,
consistent with the reported S-RNase expression. In contrast, the
S63 sequence was present in all plants examined and was
ubiquitously expressed in different organs and bud developmental
stages. Genetic analysis confirmed the association with self-
incompatible function in five S-RNase candidates while S5 and S63
appeared to be nonfunctional relic S-RNases. Phylogenetical
analysis showed the Nicotiana alata S-RNases were included in
eight transgeneric lines confirming that this species represents a
broad sample of allelic variation at the S-locus of the Solanaceae.
