Biodistribution and phototherapeutic properties of Zinc (II) 2,9,16,23-tetrakis (methoxy) phthalocyanine in vivo

E. INES YSLAS; CESAR PRUCCA; SILVIA ROMANINI; EDGARDO N. DURANTINI; MABEL BERTUZZI; VIVIANA RIVAROLA

ELSEVIER SCIENCE BV

Año: 2009 vol. 6 p. 62 - 62

Photodynamic therapy (PDT) was investigated using the phthalocyanine photosensitizer Zinc (II) 2,9,16,23-tetrakis (methoxy) phthalocyanine (ZnPc(OCH3)4) on BALB/c mice. Animals bearing tumor were treated with 0.2 mg/kg body weight (bw) ZnPc(OCH3)4 and 24 h later were irradiated with 70, 140 and 210 J/cm2 of visible light from a source delivering 39mW/cm2. In this study, we have tested the efficiency of ZnPc(OCH3)4 liposomal formulation on mice. Biodistribution studies were performed in tumor-free mice and tumor-bearing mice at various time points up to 24 h after ZnPc(OCH3)4-PDT treatment. The tumor sizes were evaluated over different period in parallel experiments. The maximal efficiency and selectivity of photosensitizer accumulation in tumor tissue take place at 24 h after drug administration of 0.2 mg/kg bw ZnPc(OCH3)4. In the tumor sections for biochemical studies, apoptosis was visualized by activation of caspase-3. ZnPc(OCH3)4-PDT tumors showed a significant delay in growth