Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

VELAZQUEZ, FABIOLA N.; MIRETTI, MARIANA; BAUMGARTNER, MARIA T.; CAPUTTO, BEATRIZ L.; TEMPESTI, TOMAS C.; PRUCCA, CÉSAR G.

Scientific Reports

Springer

Año: 2019 vol. 9

lioblastoma multiforme is considered to be one of the most aggressive types of tumors of the centralnervous system, with a poor prognosis and short survival periods of ~ one year. The current protocolfor glioblastoma treatment includes the surgical excision of the primary tumor followed by radio andchemotherapy. Photodynamic therapy (PDT) is considered a promising strategy for the treatmentof several types of tumors. Phthalocyanines (Pcs) are good photosensitizers (PSs) for PDT becausethey induce cell death in several cellular models. ZnPc (Zn(II)phthalocyanine) is a well-known Pc,extensively tested in different cells and tumor models, but its evaluation on a glioblastoma modelhas been poorly studied. Herein, we compare the capacity of ZnPc and one of its derivatives, Zn(II)tetraminephthalocyanine (TAZnPc), to photoinactivate glioblastoma cells (T98G, MO59, LN229 andU87-MG) in culture. We measured the cellular uptake, the toxicity in the dark and the subcellularlocalization of the dif