Temporal regulation of tumor growth in nocturnal mammals: In vivo studies and chemotherapeutical potential

WAGNER, PAULA M.; PRUCCA, CÉSAR G.; VELAZQUEZ, FABIOLA N.; SOSA ALDERETE, LUCAS G.; CAPUTTO, BEATRIZ L.; GUIDO, MARIO E.

FASEB JOURNAL

FEDERATION AMER SOC EXP BIOL

Año: 2021 vol. 35

0892-6638

umors of the nervous system including glioblastoma multiforme (GBM) are the most frequent and aggressive form of brain tumors; however, little is known about the impact of the circadian timing system on the formation, growth, and treatment of these tumors. We investigated day/night differences in tumor growth after injec- tion of A530 glioma cells isolated from malignant peripheral nerve sheath tumor (MPNSTs) of NPcis (Trp53+/−; Nf1+/−) mice. Synchronized A530 cell cultures ex- pressing typical glial markers were injected at the beginning of the day or night into the sciatic nerve zone of C57BL/6 mice subject to a 12:12 hours light/dark (LD) cycle or after being released to constant darkness (DD). Tumors generated in animals injected early at night in the LD cycle or in DD showed higher growth rates than in animals injected diurnally. No differences were found when animals were injected at the same time with cultures synchronized 12 hours apart. Similar experiments perform