CHARACTERIZATION OF Tritrichomonasfoetus EXCRETION/SECRETION ANTIGENS

ABDALA, ME; RIVERO, MB; LUQUE, ME; CARRANZA PG; PRUCCA, CG; RIVERO, FD

Parana

Congreso; 54 Reunion Anual de la Sociedad Argentina de Bioquímica y Biología Molecular; 2018

Sociedad Argentina de Bioquímica y Biología Molecular

Bovine trichomonosis, a disease of the bovine urogenital tract, is caused by the protozoan Tritrichomonasfoetus (Tf). It causes endometritis,infertility and premature death of the embryo, which generates considerable economic losses. Have a worldwide distribution and in Argentina it´sconsidered endemic. Excretion/secretion antigens (AES) have been shown to be virulence factors in both Trichomonas vaginalis (Tv), Tfhomolog in humans, and in other organisms and constitute a target for the development of diagnostic methods and vaccines. The objective was todetermine the AES repertoire of Tf. Six isolates were incubated with buffer TIB to perform AES purification and then were filtered andconcentrated by ultracentrifugation. An SDS-PAGE was performed and then sent for analysis by MALDI-TOF. The data obtained were analyzedand compared against the Tf and Tv genomes. The results showed that there is a low homology between AES of Tf vs Tv, however, a highhomology was observed between the different Tf isolates. The proteins present in AES of Tf were classified functionally, showing among othersthe following groups: posttranslational modification proteins (14%), binding proteins (12%), vesicular transport proteins (11%) and unknownfunction proteins (18%). In conclusion: a) There are great similarities between the AES of different Tf isolates, which allows to identify theprofile of excretion/secretion. b) The differences with Tv allow us to infer certain specificity of the AES of Tf c) According to the diverseproteins found, different trials can be approached to evaluate new targets for diagnosis and treatment of this disease.