Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical Monocytes

Cytometry Part A

Attila Tárnok

Año: 2014

1552-4930

n this article, we present a flow cytometry assay by which human blood monocyte sub-populations?classical (CD1411CD162), intermediate (CD1411CD161), and non-classical (CD141CD1611) monocytes?can be determined. Monocytic cells were selected from CD451 leukocyte subsets by differential staining of the low-density lipo-protein receptor-related protein 1 (LRP1), which allows reducing the spill-over of natu-ral killer cells and granulocytes into the CD161 monocyte gate. Percentages of monocyte subpopulations established by this procedure were significantly comparable with those obtained by a well-standardized flow cytometry assay based on the HLA-DR monocyte-gating strategy. We also demonstrated that LRP1 is differentially expressed at cell surface of monocyte subpopulations, being significantly lower in nonclassical monocytes than in classical and intermediate monocytes. Cell surface expression of LRP1 accounts for only 20% of the total cellular content in each monocyte subpopula-tion. Fi