LRP1 expression and function in human blood peripheral monocyte (bpm) subpopulations.

Congreso; XLVIII Reunión Anual - Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2012

LRP1 EXPRESSION AND FUNCTION IN HUMAN BLOOD PERIPHERAL MONOCYTE (BPM) SUBPOPULATIONS. Ferrer DG, Jaldín Fincati JR, Sánchez MC, Chiabrando GA. CIBICI-CONICET, Depto Bioquímica Clínica Facultad de Ciencias Químicas, Univ Nac Córdoba. E-mail: dferrer@bioclin.fcq.unc.edu.ar Alpha2-Macroglobulin (a2M*) is recognized by LDL receptor-related protein 1 (LRP1), and induces the cell migration of macrophage. BPM are involved in the development of atherosclerosis. In human, at least three subpopulations of BPM may be identified (classical, intermediate and non-classical). LRP1 it has been associated with atherosclerotic processes. The LRP1 expression and function in BPM subpopulations is not well established. By flow cytometry and confocal microscopy we characterized the LRP1 expression in BPM subpopulations. By two-dimensional wound scratch assay we examined the cell migration of each BPM subpopulation isolated by cell sorter and cultured in presence of a2M*, a LRP1 ligand. LRP1 was three-fold more expressed in classical than in intermediate and non-classical BPM subpopulations. In this subpopulation, LRP1 was mainly localized at perinuclear regions, increasing the cell surface expression under 2M* stimulation for 30 min). In this time, the classical BPM also formed cellular protrusions with accumulation of F-actin and LRP1, which correlated with an increased cell migration. Thus, our data demonstrate classical BPM subpopulation are induce to migrate by the a2M*/LRP1 interaction, which may be involved with the development of atherosclerosis.