Transcriptional regulation of tocophetol biosyntesis in tomato

LEANDRO QUADRANA; JULIANA ALMEIDA; SANTIAGO N. OTAIZA; TOMAS DUFFY ; JUNIA V. CORRÊA DA SILVA; FABIANA DE GODOY; RAMON ASÍS; LUISA BERMÚDEZ; ALISDAIR R. FERNIE; FERNANDO CARRARI; MAGDALENA ROSSI

SPRINGER

Lugar: Berlin; Año: 2013 p. 309 - 309

ocopherols, compounds with vitamin E (VTE) activity, are potent lipid-soluble antioxidants synthesized only by photosynthetic organisms. Their biosynthesis requires the condensation of phytyl-diphosphate and homogentisate, derived from the methylerythritol phosphate (MEP) and shikimate pathways (SK), respectively. These metabolic pathways are central in plant chloroplast metabolism and are involved in the biosynthesis of important molecules such as chlorophyll, carotenoids, aromatic amino-acids and prenylquinones. In the last decade, few studies have provided insights into the regulation of VTE biosynthesis and its accumulation. However, the pathway regulatory mechanism/s at mRNA level remains unclear. We have recently identified a collection of tomato genes involved in tocopherol biosynthesis. In this work, by a dedicated qPCR array platform, the transcript levels of 47 genes, including paralogs, were determined in leaves and across fruit development. Expression data were analyzed fo