OTAIZA GONZÁLEZ SANTIAGO NICOLÁS
Congresos y reuniones científicas
Título:
Functional characterization of a Sec14-like protein involved with tomato fruit tocopherol content
Autor/es:
BERMUDEZ L; DE GODOY F; OTAIZA S; ASIS R; CARRARI; ROSSI M
Lugar:
Puerto Madryn, Chubut.
Reunión:
Congreso; XLVI Reunión Anual - Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.; 2010
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.
Resumen:
Tomato (Solanum lycopersicum) is an important food crop worldwide and represents an excellent model plant for genomics and fruit metabolism research. Numerous wild related species have demonstrated to be an untapped source of valuable genetic variability including nutritional and industrial quality traits. Based on metabolic QTL mapping data from a S. pennellii introgression lines population, our group has identified a SEC14-like protein co-localizing with a α-tocopherol QTL on chromosome 9 (segment 9BDE). SEC14-like proteins have been identified in all kingdoms and showed to be involved in membrane trafficking, regulation of phospholipid metabolism and phosphoinositide signaling pathways. Sequence analyses revealed amino acid polymorphisms between the S. lycopersicum and S. pennellii alleles. Moreover, expression profiles in different tomato tissues also showed differential expression levels in mature fruits. Sub-cellular localization experiments showed that this protein is targeted to chloroplasts. Analyses of iRNA plants suggest impairment in the photosynthetic performance in silenced plants. These results underpin the involvement of the SEC14-like protein in tomato fruit tocopherol content.
