REDOX REGULATION OF AUTOPHAGY: EFFECTS OF DIFFERENT ROS

ENET A; ROBERT G; SAAVEDRA-BORELLI LS; OTAIZA GONZÁLEZ SN; BELLAGIO T; FINELLO J; LASCANO HR

Córdoba

Congreso; XXXII Reunión Argentina de Fisiología Vegetal (RAFV) y XVI Congreso Latinoamericano de Fisiología Vegetal; 2018

Sociedad Argentina de Fisiología Vegetal

Autophagy is a major self-degradative process highly conservedin eukaryotic cells, with fundamental roles in cellularand organism homeostasis. During this process, newlyemerging double-membrane vesicles called autophagosomesinclude protein or whole organelles, and deliver themto the vacuole for their degradation. Diverse situations ofstress have been shown to be inducers of the autophagicprocess, being the different ROS and its site of productionmodulators of this process. One of the major source of ROSin the plant cell is the chloroplast, where different oxygenspecies can be produced. The aim of this work is to studythe effects on the autophagic processes of the differentROS produced in the chloroplast, such as peroxide (H2O2),superoxide (O2?) and singulet oxygen (1O2). Our preliminaryresults suggest that the production of O2? or H2O2 inthe chloroplast has an inhibitory effect on the autophagy,where treatments with different scavengers rescued thisinhibition. Also, the production of 1O2 shows an increaseof the autophagy flux, suggesting a opposite roll of the differentROS in the autophagy process.