Direct and simultaneous observation of transcription and chromosome architecture in single cells with Hi-M

CARDOZO GIZZI AM; ESPINOLA, SERGIO M.; GURGO, JULIAN; HOUBRON, CHRISTOPHE; FICHE, JEAN-BERNARD; CATTONI, DIEGO I.; NOLLMANN, MARCELO

NATURE PROTOCOLS

NATURE PUBLISHING GROUP

Lugar: Londres; Año: 2020

1754-2189

p dir="ltr" style="line-height: 1.38; text-align: justify; margin-top: 0pt; margin-bottom: 0pt;" id="docs-internal-guid-eb4f9624-7fff-edea-7f9c-82f91487c790">Simultaneous observation of 3D chromatin organization and transcription at the single cell level and with high spatial resolution may hold the key to unveil the mechanisms regulating embryonic development, cell differentiation and even disease. We have recently developed Hi-M, a technology that allows for the sequential labelling, 3D imaging and localization of multiple genomic DNA loci together with RNA expression in single cells within whole, intact