Semi-interpenetrated, dendritic, dual-responsive nanogels with cytochrome C corona induce controlled apoptosis in HeLa cells

MICELI, ENRICO; WEDEPOHL, STEFANIE; OSORIO BLANCO, ERNESTO ; RIMONDINO, GUIDO NOÉ; MARTINELLI, MARISA; STRUMIA, MIRIAM C; MOLINA, MARIA; KAR, MRITYUNJOY; CALDERON, MARCELO

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2018 vol. 130 p. 115 - 115

0939-6411

he use of thermoresponsive nanogels (NGs) allows the controlled release of therapeutic molecules upon thermal switch. Usually, this strategy involves the use of temperature increase to activate cargo expulsion from shrunken NGs. In this study, poly(N-isopropylacrylamide (pNIPAM)-based NGs were involved in the release of a therapeutic protein corona by temperature decrease. NGs based on dendritic polyglycerol (dPG) and thermoresponsive pNIPAM NGs were semi-interpenetrated with poly(4-acryloylamine-4-(carboxyethyl)heptanodioic acid) (pABC). The resulting semi-interpenetrated NGs retain the thermoresponsive properties of pNIPAM, together with pH-responsiveness of dendritic pABC, as a secondary network, in one single nanoparticle. Semi-interpenetrated polymer network (SIPN) NGs are stable in physiological conditions, exhibit a reversible phase change at 33 °C, together with a tunable ζ-potential in the range of 0 to -18 mV around the body temperature. A cytochrome c (cyt c) corona wa