Interaction of polypyridyl Cr(III) complexes with bovine serum albumin

GARCIA, PABLO F.; CORONEL ARRECHEA, CONSUELO; VELO, ALEJANDRA; RIVA, JULIETA; YUDI, LIDIA M.; ARGÜELLO, GERARDO A.

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

SPRINGER

Año: 2020 vol. 49 p. 125 - 125

0175-7571

e report a detailed investigation of the interaction of Cr(NN)33+ with bovine serum albumin (BSA), an important protein for the transport of drugs in blood plasma which allows us to understand further the role of Cr(NN)33+ as a sensitizer in photodynamic therapy (PDT). Chromium(III) complexes, Cr(5Cl-phen)33+, Cr(5Me-phen)33+ and Cr(5Ph-phen)33+ (where Cl = chlorine, Me = methyl and Ph = phenyl are substituents in position 5 of the phen = 1,10-phenanthroline bidentate ligand), were used for the present study. The interactions of BSA with Cr(NN)33+ were assessed employing fluorescence spectroscopy and UV?Vis absorption spectroscopy; in addition electrochemical experiments carried out at a liquid/liquid interface gave insight into the relative hydrophobicities of the complexes. We found that chromium complexes bind strongly with bovine serum albumins (BSA) with intrinsic binding constants, Kb, of (3.33 ± 0.08) × 105